Folic acid - test

• Alternative names
• Definition
• How the test is performed
• How to prepare for the test
• How the test will feel

• Why the test is performed
• Normal Values
• What abnormal results mean
• What the risks are
• Special considerations

Alternative names   

Definition    Return to top

The folic acid test measures the amount of folate in the blood.

How the test is performed    Return to top

Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic. An elastic band is placed around the upper arm to apply pressure and cause the vein to swell with blood.

A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the band is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

In infants or young children:

The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. A bandage may be applied to the puncture site if there is any bleeding.

How to prepare for the test    Return to top

Fast for 6 hours before the test. The health care provider should be notified if you are using any drugs that can affect test results, including folic acid supplements (see Special considerations).

Infants and children:

The preparation you can provide for this test depends on your child's age, previous experiences, and level of trust. For general information regarding how you can prepare your child, see the following topics:

• Infant test or procedure preparation (birth to 1 year)
• Toddler test or procedure preparation (1 to 3 years)
• Preschooler test or procedure preparation (3 to 6 years)
• Schoolage test or procedure preparation (6 to 12 years)
• Adolescent test or procedure preparation (12 to 18 years)

How the test will feel    Return to top

When the needle is inserted to draw blood, you may feel moderate pain, or only a prick or stinging sensation. Afterward, there may be some throbbing.

Why the test is performed    Return to top

This test is used to evaluate for folic acid deficiency. It can be performed on either whole blood or red blood cells, but measuring folate in red blood cells is more accurate.

Folic acid -- one of the B vitamins -- is necessary for synthesis of nucleotides, which are the building blocks of DNA. A deficiency of DNA synthesis can most easily be seen in rapidly-dividing cells, such as immature red blood cells. Red blood cells that are formed in the presence of insufficient folic acid have shortened life spans, are larger than normal, and have reduced ability to carry oxygen.

Normal Values    Return to top

The normal range is 2.7 - 17.0 ng/ml (nanograms per milliliter)

What abnormal results mean    Return to top

Lower-than-normal levels may indicate:

• Inadequate diet
• Malabsorption syndrome (for example, sprue and celiac disease)
• Malnutrition

• Anemia of folate deficiency
• Megaloblastic anemia

What the risks are    Return to top

• Excessive bleeding
• Fainting or feeling light-headed
• Hematoma (blood accumulating under the skin)
• Infection (a slight risk any time the skin is broken)
• Multiple punctures to locate veins

Special considerations    Return to top

Pregnancy increases the need for folic acid.

Drugs that can decrease folate measurements include alcohol, aminosalicylic acid, birth control pills, estrogens, tetracyclines, ampicillin, chloramphenicol, erythromycin, methotrexate, penicillin, aminopterin, phenobarbital, phenytoin, and antimalarials.

Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.